https://www.gelfandlab.org/link-gallery daily 0.75 2020-01-31 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579785038920-M120JWQ5Q7ZJE4R86PKM/NU+logo.gif LINK Gallery - Northwestern University https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579785038920-M120JWQ5Q7ZJE4R86PKM/NU+logo.gif LINK Gallery - Northwestern University https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579966819642-3OEMB2K4FTXJM0BOYWOC/Screen+Shot+2020-01-25+at+9.37.00+AM+copy.jpg LINK Gallery - Department of Cell and Developmental Biology https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579966896535-C049LG1RRZU02166N7B6/CAM.jpg LINK Gallery - Center of Advanced Microscopy https://cam.facilities.northwestern.edu/ https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579968573519-3JCZ5RYEUWB6DWLPQDAW/November2019Poster.jpg LINK Gallery - Chicago Cytoskeleton Meeting https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579967024029-9DVVAC44LU398PIPJUWO/NIH.png LINK Gallery - NIH https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580431496279-WMDLBDI8Y0KBYDPGQ32M/pubmed-meta-image.png LINK Gallery - Pubmed https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580431592819-XLQWC0B0TRDIUKYMWH6C/ASCB.png LINK Gallery - American Society of Cell Biology https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580431763739-VGRQ0T4OYIIKFHZQGW7C/Flybase.jpg LINK Gallery - FlyBase https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580431898199-CON6PCH3AQKNR3M30HTJ/bloominton.jpg LINK Gallery - Bloomington Drosophila Stock Center https://www.gelfandlab.org/members-gallery daily 0.75 2026-03-23 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579785808406-2XLSCZYSFDE7JGIZQXWA/20150720-DSC_4091.jpg Members gallery - Vladimir I. Gelfand, PhD (Principal Investigator) Leslie B. Arey Professor of Cell, Molecular and Anatomical Sciences email: vgelfand@northwestern.edu https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579785808406-2XLSCZYSFDE7JGIZQXWA/20150720-DSC_4091.jpg Members gallery - Vladimir I. Gelfand, PhD (Principal Investigator) Leslie B. Arey Professor of Cell, Molecular and Anatomical Sciences email: vgelfand@northwestern.edu https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1742415216662-F6WHWW5Z2FVLB5ZJZUJI/Lu_Wen_PhD_20220701_MPP19250-small.jpg Members gallery - Wen Lu (Research Assistant Professor) Received her bachelor degree in Biological Sciences at Peking University (China) and her PhD degree in Genetics at the University of Chicago. Her undergrad thesis focused on expression and characterization of the Spike protein of SARS-COV virus in insect cells and mammalian cells; her doctoral thesis investigated the role of the small GTPase Rac in the germ line stem cell asymmetric cell division and self-renewal. In the Gelfand lab, Wen is interested to understand how the cytoskeletal components polarize large cells, such as neurons, and oocytes, in the model organism Drosophila melanogaster (AKA fruit flies). https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580423291157-S0F9VOPN06Q1B0SHDWBF/Cell.jpg Members gallery - Anyuta Serpinskaya (aka Anna Gelfand), PhD (Research Specialist) email: a-gelfand@northwestern.edu https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580423364079-0PLQVJ35EQDKSB2NL57W/Cell.jpg Members gallery - Margot Lakonishok, PhD (Research Specialist) email: m-lakonishok@northwestern.edu https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1648235131934-L2RY99CPN1W9XE67EE6W/MAR_0692%7E3.JPG Members gallery - Bhuvanasundar Ranganathan (Research Associate) Bhuvan obtained Ph.D. in biological sciences in 2016, from the Birla Institute of Technology and Science in Pilani, India. Doctoral work is focused on the biochemical and biophysical characterization of the enzyme Lysyl oxidase (LOX). Then moved to the National University of Singapore (NUS), Singapore in 2017, where he worked as a post-doctoral fellow and studied the anti-angiogenic properties of ADAMTS5 protein. Currently studying the dynamics of vimentin intermediate filament. Towards that, exploring how vimentin filaments interact with other cytoskeletal components such as microtubule, actin, and motor proteins for its dynamics. He enjoys playing with a microscope and taking images. email: bhuvan@northwestern.edu https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1742412723510-O8IDPV4Q2PM6OUIMLWWL/Shannon.jpg Members gallery - Shannon Liang (Research Technologist) A Chicago native, Shannon earned her B.S. in Molecular, Cellular, and Developmental Biology from the University of Michigan in Ann Arbor. Her undergraduate thesis focused on identifying domains of NOTCH1-collaborating transcription factor Ets1 that are important in T-ALL. Her project in the Gelfand lab aims to elucidate the role of microtubules during tracheal development in Drosophila. When she is not taking care of her flies, Shannon enjoys working out, playing the violin, and sewing. https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1774296914265-VBWOUZ2PNS0U54HX0RY2/FionaAnn.jpg Members gallery - Fiona Ann Nicdao - Research Technologist https://www.gelfandlab.org/new-index-1 daily 0.75 2025-06-09 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580339264801-CGX2S935XG9W51OZPJFP/Screen+Shot+2020-01-29+at+5.06.40+PM.png New Index Model of microtubule-microtubule sliding driven by kinesin-1. Kinesin-1 slides antiparallel microtubules apart with their minus-ends leading (left panel). When kinesin-1 binds to parallel microtubules (right panel), forces applied by the two motors to the two microtubules are counteracted resulting in no net movement; instead, kinesin-1 crosslinks these microtubules. Large green arrows indicate direction of microtubule sliding; small orange arrows indicate the direction of kinesin-1 movement relative to microtubules. https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579915293476-HHRIW36VVBB1MO4VQXME/JCB+cover.jpg New Index During late oogenesis, Drosophila oocyte cytoplasm undergoes circular movement called ooplasmic streaming. Posterior determinants containing the fluorescently labeled marker Staufen move with the cytoplasmic flow, but dramatically slow down near the posterior pole in a wild-type oocyte (right) but not in an oocyte expressing a dominant-negative form of myosin V (left). Spectrum-colored tracks represent the movement of Staufen-containing particles within a 10-min period of time. https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580403652677-TO95ACT7DHF3DY0XBTGM/Transport+of+VIF+in+RPE+Cells.gif New Index https://www.gelfandlab.org/new-events daily 0.75 2020-01-23 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579752005718-1QT2QVFPLE7IJU1VND62/Screen+Shot+2020-01-22+at+9.58.23+PM.jpg New Events https://www.gelfandlab.org/alumni-1 daily 0.75 2026-03-23 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1609979596763-Q2O7HNBZXSZ1U7GWVPYH/Untitled.jpg Alumni - Urko del Castillo, PhD Associated with Gelfand’s lab from 2011 to 2020 as Post doctoral research fellow. Currently, he is working as Research Associate at Weill Cornell Medicine, Division of Hematology and Medical Oncology, New York. https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1609979596763-Q2O7HNBZXSZ1U7GWVPYH/Untitled.jpg Alumni - Urko del Castillo, PhD Associated with Gelfand’s lab from 2011 to 2020 as Post doctoral research fellow. Currently, he is working as Research Associate at Weill Cornell Medicine, Division of Hematology and Medical Oncology, New York. https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1596995645074-A2LJF4BWLV21HEGFXOTS/Cell.jpg Alumni - Rosalind Norkett, PhD Associated with Gelfand’s lab from 2016 to 2020 as post doctoral fellow. Currently, senior research scientist at Francis crick institute in London, England https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580829599751-A4B7HNQKKRSHQIQRY2M6/Cell.jpg Alumni - Jonathan R. Brent, MD-PhD (Research Fellow) Jonathan is interested in translating discoveries underlying the basic mechanisms of motor neuron disease into treatments for the disease. His current research focuses on the role of cytoskeletal dynamics and axon transport in diseases such as Amyotrophic Lateral Sclerosis and Hereditary Spastic Paraplegia. He received his MD.PhD. at Columbia University College of Physicians and Surgeons where his doctoral thesis investigated the convergent roles of TDP-43 and FUS/TLS in ALS. Later, he came to Northwestern Memorial Hospital where he pursued Neurology Residency and Neuromuscular Medicine Fellowship. He was awarded the NIH R25 research training fellowship. He is currently Instructor of Neurology at Northwestern Feinberg School of Medicine. email: jonathan.brent@northwestern.edu https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1683060656819-Y1F1T7TQNL4382JPV0N1/Cell.jpg Alumni - Sun Kim (Graduate candidate) I joined the Gelfand lab in June of 2022. I am interested in understanding how microtubules are spatiotemporally regulated during oogenesis using Drosophila melanogaster as a model system. I live with two furry family members, Kimchi and Somang, from South Korea. I enjoy cuddling with my dogs, cooking, and reading (non-scientific books) in my free time. https://www.gelfandlab.org/gallery daily 0.75 2026-03-23 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1774297474472-9IL7H4HQPTEA7WJ9XSAH/groupFeb2026.png Gallery https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1661359264418-4MG906QVYIUHWIGCMJZD/image.jpg Gallery - Cleaned the LAB and ready for Volodya sponsored lung https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1774297474472-9IL7H4HQPTEA7WJ9XSAH/groupFeb2026.png Gallery https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1774297522369-ED4EPUGKSZZQP07H6G2L/IMG_3759.JPG Gallery https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1774297534433-G66GU1YY5GJUIDEVMITY/IMG_5471.jpeg Gallery https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1774297549314-EN7W6NLTH0WVVPIIJ08L/IMG_5472.jpeg Gallery https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1749427831941-XGBGWDC4D2S5KC2307LD/image-asset.jpeg Gallery - 2025 Spring https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1736283978078-W9CWEDM17U9SIYAOLCYB/image-asset.jpeg Gallery - V Birthday 2025 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1736284041287-8ZC5W1YTG9AX2SYWT83D/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1736283637684-IFXGXW2N06GA876O3Q5D/image-asset.jpeg Gallery - Volodya Birthday 2025 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1736284090695-RP3CGX4IIXJR92U1AAI7/image-asset.jpeg Gallery https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1728591004578-7BDXC38CLPY2UY2S6ARU/image-asset.jpeg Gallery - Lunch with Michael Winding and his lab members Michael Winding, a former Gelfand graduate student and current PI at The Francis Crick Institute in London, visited our lab. It was a pleasure meeting his lab members and enjoying a great lunch https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1723840050022-V49GEHJ62E2URXTTTHHN/image-asset.jpeg Gallery - Lab Lunch with MinJong Park and Hanako Drechsel Lab alumni Dr. MinJong Park visited and Farewell for summer intern Hanako Drechsel. https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1723840226910-HS7KBZSD3EEUDM9J1Z7X/1000052159.jpg Gallery - Summer is not complete without a fresh lab pic https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1704733564245-Y48S1JNQJZ5YM63JF6VE/PXL_20240105_211710355.TS_exported_6102_1704508097048.jpg Gallery - Volodya's Birthday celebration 2024 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1704734917927-CRVBTYXRUOEM0SB8GJJ8/PXL_20240105_210906192.RAW-01.COVER.jpg Gallery - Lab full of Volodya https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1704935220477-7WOCND0QAOOVGI6CYB4B/image.jpg Gallery - Can you spot the original volodya? https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1702386681634-02M2S6J2Z29OHMV3VLR3/image-asset.jpg Gallery - Scientific Clans of Vladimir Gelfand Alumni and current members of Gelfand lab enjoyed a memorable night in Boston, 2023 @ ASCB conference https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1702336269392-NRGVRXDLZCF7B4LPIPNR/image-asset.jpeg Gallery - ASCB talk by Bhuvan Bhuvanasundar Renganathan gave a talk in ASCB subgroup session on intermediate filament @ASCB cellBio2023 at Boston https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1702388518078-BK45MR0Z77FCPTAWOMW1/image-asset.jpg Gallery - Wen Poster presentation Wen explaining her poster during ASCB CellBio2023 @ Boston https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1702336449760-4ZCTVOHJI0XL3AT0PW50/image-asset.jpeg Gallery - Sun Poster presentation Sun explaining her poster during ASCB CellBio2023 @ Boston https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1702336414090-BW79IX61JEACEGYUKM34/image-asset.jpeg Gallery - Bhuvan Poster presentation Bhuvan explaining his poster during ASCB CellBio2023 @ Boston https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1683043924228-RW10FP3FE3YHGM6OJYPG/IMG-20230427-WA0003.jpg Gallery https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1661378699975-32G5N9YPMFVK4Q0NJED6/Screen+Shot+2022-08-24+at+4.29.34+PM.png Gallery - Crushing COVID-19 lockdown time with virtual lab meetings -2020 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1661378725650-LDW6U0IZBJ7GVFJNBNU1/PXL_20220705_183626190%7E2.jpg Gallery - Lab Spring cleaning done - Prepared for Volodya sponsored lunch https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1661379209435-TAKQYU0AB7TM737CLR7A/IMG-20210903-WA0001.jpg Gallery - Bidding farewell to ROZ https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1661378676959-TNOYSK6CL21SXA9SIZAO/Gelfand+Lab_2009_06_06.jpg Gallery - Get Together - Barbecue dinner https://www.gelfandlab.org/welcome daily 1.0 2020-01-22 https://www.gelfandlab.org/publications daily 0.75 2025-03-13 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579742929706-BKZS0U4CLLQF7AHJ3N94/Screen+Shot+2020-01-22+at+5.06.23+PM2.jpg Publications https://www.gelfandlab.org/contact-us daily 0.75 2023-05-18 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579732720418-CTXB5YV7BCXWDCFFLZYT/Storm.jpg Contact us https://www.gelfandlab.org/neurite-outgrowth daily 0.75 2020-01-30 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579738647801-ATROXPZ608253I46JIQW/Adam%27s_spreading_1.gif Neurite Outgrowth https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580339264801-CGX2S935XG9W51OZPJFP/Screen+Shot+2020-01-29+at+5.06.40+PM.png Neurite Outgrowth Model of microtubule-microtubule sliding driven by kinesin-1. Kinesin-1 slides antiparallel microtubules apart with their minus-ends leading (left panel). When kinesin-1 binds to parallel microtubules (right panel), forces applied by the two motors to the two microtubules are counteracted resulting in no net movement; instead, kinesin-1 crosslinks these microtubules. Large green arrows indicate direction of microtubule sliding; small orange arrows indicate the direction of kinesin-1 movement relative to microtubules. https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580082988427-PNDPOU326K8F3U7TPUC8/Sequence+01_4.gif Neurite Outgrowth Sliding Microtubules Push against the Neurite Tips and induce neurite outgrowth. Young neurons (<3 hr after plating) from embryos expressing GFP-microtubules and cell membrane was stained by Deep Red dye. Scale bar represent 5 μm. https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580081737278-PCJENU2WYCUWKTIZJNVC/PAvarotti+video.gif Neurite Outgrowth Pav-KLP (Kinesin-6) depletion Stimulates Microtubule Sliding in Drosophila S2 Cells, Time-lapse images of photoconverted microtubules in control (left panel) or Pav-KLP RNAi (right panel) of S2 cells expressing tdEOS-αtubulin. https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580336420331-E67URIZZJV1XLRJP4V4G/Screen+Shot+2020-01-29+at+4.19.46+PM.png Neurite Outgrowth Sensory dendritic arborisation neurons in Drosophila third instar larvae. Depleting levels of the motor protein Pav-KLP or the kinase Trc increases microtubule sliding. This leads to increased neurite outgrowth and an overdeveloped dendritic arbor seen here https://www.gelfandlab.org/team daily 0.75 2024-01-16 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1705427167190-ZWYIUW03BNSZ5OF0VFQC/PXL_20240105_210906192.RAW-01.COVER.jpg Team https://www.gelfandlab.org/research-projects daily 0.75 2020-08-07 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579706872938-J3SILC3XSXHDVBCU2YMC/superesolution+1.jpg Research Projects https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579732240456-OO03OJQOVKVCHN1FJ490/Adam%27s_spreading_1.gif Research Projects - NEURITE OUTGROWTH https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579732271080-DJNSB5HH7O5QCELJJYYS/Nos-Staufen-SunTag+example+2.gif Research Projects - POLARITY DETERMINATION https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579732307377-HV97JVK4Y067XZIH9VZ9/Merged-224.gif Research Projects - DYNAMICS OF INTERMEDIATE FILAMENTS https://www.gelfandlab.org/links daily 0.75 2020-01-25 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579999391278-2F07YV58UF2E6ZU42EXC/Wendella_WineandCheese_UrkodC_Website+Resolution-001-5+copy.jpg Links https://www.gelfandlab.org/dynamics-of-intermediate-filaments daily 0.75 2020-01-31 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580403433266-T0K1RADSLYAQ6BZIF3FM/Merged-224.gif Dynamics of intermediate filaments https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580403652677-TO95ACT7DHF3DY0XBTGM/Transport+of+VIF+in+RPE+Cells.gif Dynamics of intermediate filaments https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580404066579-SMDRMOD25CALXR2GACVM/Merged-224.gif Dynamics of intermediate filaments https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1683043123550-ESD3N7WSF0EEY93UZKDU/Untitled-3.jpg Dynamics of intermediate filaments - Vimentin aggregates in GAN KO cells Inhibited transport of intermediate filaments is the underlying reason for their aggregation in the Giant axonal neuropathy disorder. Distribution of vimentin and microtubule in control (upper cell) and GAN KO (lower) cells https://www.gelfandlab.org/polarity-determination daily 0.75 2020-01-25 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579814188376-RENIXX8L8CTKHP2D7HJK/Nos-Staufen-SunTag+example+2.gif Polarity determination https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579915293476-HHRIW36VVBB1MO4VQXME/JCB+cover.jpg Polarity determination During late oogenesis, Drosophila oocyte cytoplasm undergoes circular movement called ooplasmic streaming. Posterior determinants containing the fluorescently labeled marker Staufen move with the cytoplasmic flow, but dramatically slow down near the posterior pole in a wild-type oocyte (right) but not in an oocyte expressing a dominant-negative form of myosin V (left). Spectrum-colored tracks represent the movement of Staufen-containing particles within a 10-min period of time. https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579915361810-0JJLCJ6U9V2AWCMX4UWD/Microtubules+at+the+ring+canals+caption.jpg Polarity determination Microtubules are connecting the nurse cells with the oocyte through the actin-rich channels (ring canals). Microtubules are labeled the GFP-tagged Patronin and F-actin is stained with Rhodamine-conjugated Phalloidin. https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579915394628-TO1P7DZFOJD5QYMXUJTV/PNAS+MT-SunTag.tif+%28RGB%29.jpg Polarity determination Stable immotile microtubules are closely associated with cortical actin in a streaming oocyte. Microtubules are labeled with the K560Rigor-SunTag system (MT-SunTag) and F-actin is stained with Rhodamine-conjugated phalloidin. https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1579915437806-KJ246AKM5TT1G4NYNME8/Streaming+in+Control+vs+MyoV+mutant.gif Polarity determination During late oogenesis, Drosophila oocyte cytoplasm undergoes circular movement called ooplasmic streaming. Posterior determinants containing the fluorescently labeled marker Staufen move with the cytoplasmic flow, but dramatically slow down near the posterior pole in a wild-type oocyte (left), but not in an oocyte expressing a dominant-negative form of myosin V (right) . Scale bar, 50 µm. https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1610309651624-9H6DWNZ2BVMIG3ILV4KV/EB1-GFP+comets+in+the+nurse+cell+%28left%29+and+in+the+oocyte+%28right%29_1.gif Polarity determination - EB1-GFP comets in the nurse cell (left) and in the oocyte (right) https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1610559354633-8LSLXKHHNEP0DNGGAIGQ/Microtubule%2C+actin+and+DAPI+staining+in+a+Drosophila+egg+chamber.gif Polarity determination - Microtubule, actin and DAPI staining in a Drosophila egg chamber https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1610559644326-GXBTW5A7PJ4XIC9QUIQW/Mitochondria+movement+from+the+nurse+cells+to+the+oocyte_1_2.gif Polarity determination - Mitochondria movement from the nurse cells to the oocyte https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1649103129817-IFP311ZJ34M7OCF741CP/Dynein+cargo+transport.gif Polarity determination - Dynein cargo transport https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1649103143220-SE48I4848IK2OKGT6K1Q/dynein+glides+microtubules+and+drives+cytoplasmic+flow.gif Polarity determination - Dynein glides microtubules and drives cytoplasmic flow.gif https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1649103155384-PV4EHUNANTVHEDPHIKXI/Movement+of+microtubules+and+mitochondria.gif Polarity determination - Movement of microtubules and mitochondria https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1649103160487-KUQRKEPCFTSTNU50XPN4/MT+gliding+by+dynein.gif Polarity determination - MT gliding by dynein https://www.gelfandlab.org/team-1 daily 0.75 2021-01-13 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1580503007124-0HB2VD4ASZ1KJBDYN7SW/Gelfand+lab+2019-12.jpg Team (Copy) https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1585067488292-5GOQPTWW5DHVOVJFII05/Screen+Shot+2020-03-24+at+10.34.13+AM.png Team (Copy) Virtual lab meeting during the COVID-19 outbreak https://www.gelfandlab.org/new-page-1 daily 0.75 2026-03-23 https://images.squarespace-cdn.com/content/v1/5e2799cc6a6c054956c0b8b3/1702386681634-02M2S6J2Z29OHMV3VLR3/image-asset.jpg Gallery https://www.gelfandlab.org/image-collections daily 0.75 2023-12-12 https://www.gelfandlab.org/news-and-events daily 0.75 2024-09-06